Journal: PLoS ONE

Article Title: Novel In Vivo Imaging Analysis of an Inner Ear Drug Delivery System in Mice: Comparison of Inner Ear Drug Concentrations over Time after Transtympanic and Systemic Injections

doi: 10.1371/journal.pone.0048480

Figure Lengend Snippet: Previous bioimaging study with IVIS reported luminescence around the ear of GFAP-luc. We first reveal here that the signal results from spiral ganglion. (A) Signal observed after local injection of luciferin via tympanic membrane. (B–D) Bioluminescence image of mouse head overlaid with a corresponding micro-CT image. (B) Micro-CT image of the head. (C) Merged image of A and C. (D) Photon bioluminescence image of the head. Note the signal was overlapped with temporal bone and not with superficial skins. (E) Ex-vivo bioluminescence in a freshly dissected temporal bone. Strong signal in the cochleae was observed (arrows), while no signal was detected in the skin and ear cartridge (right). (F) Immnohistochemistry for luciferase in the cochlea showed the expression was high in spiral ganglion (arrows).

Article Snippet: An IVIS spectrum and CCD optical macroscopic imaging system (Xenogen, Alameda, CA) was used for spatiotemporal detection of the luciferase - luciferin reaction.

Techniques: Injection, Membrane, Micro-CT, Ex Vivo, Luciferase, Expressing

Journal: Journal of Immunology Research

Article Title: Role of TLR5 in the Translocation and Dissemination of Commensal Bacteria in the Intestine after Traumatic Hemorrhagic Shock

doi: 10.1155/2021/6417658

Figure Lengend Snippet: Effect of TLR5 deficiency on the translocation and dissemination of commensal bacteria. Bioluminescent C. rodentium , visualized using an in vivo imaging system (IVIS), was used to monitor bacterial translocation. (a) The panel of LB agar plates with different tissue homogenates was shown in the top left corner. Sham-operated mice or mice 48 hours after THS were inoculated by oral gavage with C. rodentium and sacrificed approximately 5 hours after the challenge. After that, sample homogenates obtained from the MLNs (b), liver (c), blood (d), and spleen (e) were plated on LB agar culture plates and grown under aerobic condition at 37°C for 12 hours. Subsequently, bacterial CFUs were visualized (a) using IVIS, and bacterial abundance was calculated (b–e). Boxes represent the interquartile range (bottom, 25 th percentile; top, 75 th percentile), and the line inside represents the median. Whiskers denote the lowest and highest values within 1.5x the interquartile range. Each group contained 8 animals. N.S. indicates not significant. All significant p values are marked, and p < 0.05 is considered significant by one-way ANOVA test. Data are representative of three independent experiments.

Article Snippet: Forty-eight hours after THS or sham operations, mice were inoculated by oral gavage with 200 μ l of a C. rodentium suspension in PBS at a density of 2.5 × 10 7 colony forming units (CFUs)/ μ l. The whole animal was imaged one hour after the bacterial challenge using a spectrum optical in vivo imaging system (IVIS) (Kodak, Rochester, USA).

Techniques: Translocation Assay, Bacteria, In Vivo Imaging

Journal: Journal of Immunology Research

Article Title: Role of TLR5 in the Translocation and Dissemination of Commensal Bacteria in the Intestine after Traumatic Hemorrhagic Shock

doi: 10.1155/2021/6417658

Figure Lengend Snippet: Effect of RA gavage on bacterial translocation in WT mice subjected to THS. Mice with or without RA treatment were sacrificed approximately 5 hours after C. rodentium challenge. Tissue samples were homogenized and cultured. Subsequently, bacterial abundance was calculated based on IVIS data. Samples were obtained from the MLNs (a), liver (b), blood (c), and spleen (d). Meanwhile, we determined the percentage of T H 1 cells 48 hours after THS in the LP after RA treatment (e, f). Bacterial translocation data are expressed as median ± interquartile range. T H 1 data are expressed as the mean ± SD, and each group contained 8 animals. All significant p values are marked, and p < 0.05 is considered significant by the one-way ANOVA test. Data represent three independent experiments.

Article Snippet: Forty-eight hours after THS or sham operations, mice were inoculated by oral gavage with 200 μ l of a C. rodentium suspension in PBS at a density of 2.5 × 10 7 colony forming units (CFUs)/ μ l. The whole animal was imaged one hour after the bacterial challenge using a spectrum optical in vivo imaging system (IVIS) (Kodak, Rochester, USA).

Techniques: Translocation Assay, Cell Culture